| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | ENAH/MENA Positive Control for STJ506420 is synthetically produced from the 710-760 sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Enah |
| Gene ID: | 13800 |
| Uniprot ID: | ENAH_MOUSE |
| Immunogen Region: | 710-760 |
| Specificity: | This is positive control is recommended for use in combination with ENAH/MENA antibody STJ506420. |
| Tissue Specificity | Expressed in heart and testis, lower levels in lung, skeletal muscle, kidney, pancreas and brain. Isoform 5 is expressed exclusively in the brain. Isoform 2 is expressed predominantly in brain, testis, ovary and fat. In the brain, isoforms 2 and 5 are expressed at highest levels in the hippocampus, cortex and midbrain, and at lowest levels in the striatum and cerebellum. Isoform 6 is expressed in brain and spleen. |
| Post Translational Modifications | NTN1-induced PKA phosphorylation on Ser-255 directly parallels the formation of filopodial protrusions. |
| Function | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. |
| Peptide Name | Protein Enabled Homolog Npc-Derived Proline-Rich Protein 1 Ndpp-1 |
| Database Links | Reactome: R-MMU-376176 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cell Projection Lamellipodium Filopodium Synapse Cell Junction Focal Adhesion Targeted To The Leading Edge Of Lamellipodia And Filopodia By Mrl Family Members Colocalizes At Filopodial Tips With A Number Of Other Proteins Including Vinculin And Zyxlin Colocalizes With N-Wasp At The Leading Edge Colocalizes With Gphn And Pfn At Synapses |
| Alternative Peptide Names | Protein Enabled Homolog protein Npc-Derived Proline-Rich Protein 1 protein Ndpp-1 protein Enah protein Mena protein Ndpp1 protein |
Information sourced from Uniprot.org