e.coli pykF protein (Recombinant) (His-Tag) (STJP017619)
SPECIFICATIONS
HostE.coli
ImmunogenE.coli
General Information
| Short Description | Recombinant-e.coli pykF-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Accession Number | NP_416191 |
| Immunogen | E.coli |
| Immunogen Region | 1-470aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIR |
Additional Info
| Background | pyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E. coli pykF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Information sourced from Uniprot.org