| Host: | E.coli |
| Applications: | SDS-PAGE |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-E.coli GroEL-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications. |
| Formulation: | Liquid in 25mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 5mM DTT, 10%Glycerol |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: | 1-548aa |
| Accession Number: | NP_418567.1 |
| Immunogen: | E.coli |
| Immunogen Sequence: | MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKIS |
| Background | GroEL protein is the major heat shock protein of E. coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E. coli and cloned into an expression vector. This protein was overexpressed in E. coli and was purified by using conventional chromatography techniques. |
Information sourced from Uniprot.org