| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-COV2 Spike protein S B.1.1.529 (Omicron)-His-Tag protein was developed from hek293 and has a target region of His-Tag. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below). NA |
| Immunogen Region: | ECD |
| Immunogen: | DNA sequence encoding extracellular fragment[16-1220] of COV2 Spike S protein (S1+S2) [accession# QIC53204.1] variant (B.1.1.529/Omicron) (A67V, del69-70, T95I, del142-144, Y145D, del211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G4 |
| Background | Recombinant NCOV Spike protein B.1.1.529 encodes the NCOV spike glycoprotein S1+S2 sequences. Recombinant COV2 Spike migrates due to glycosylation as an approximately 130 kDa protein under reducing conditions in SDS-PAGE. |
Information sourced from Uniprot.org