| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
CD8 beta Blocking Peptide for STJ505976 is synthetically produced from the 70-120 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
CD8B |
| Gene ID: |
926 |
| Uniprot ID: |
CD8B_HUMAN |
| Immunogen Region: |
70-120 |
| Specificity: |
This blocking peptide is recommended for use in combination with CD8 beta antibody, STJ505976 |
| Immunogen: |
Synthetic peptide taken within amino acid region 70-120 on human T-cell surface glycoprotein CD8 Beta chain. |
| Post Translational Modifications | Phosphorylated as a consequence of T-cell activation. Palmitoylated at the cytoplasmic tail and thereby targets the heterodimer CD8A/CD8B to lipid rafts unlike CD8A homodimers. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells. |
| Peptide Name | T-Cell Surface Glycoprotein Cd8 Beta ChainCd Antigen Cd8b |
| Database Links | Reactome: R-HSA-182218Reactome: R-HSA-198933 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinRequires The Partner Cd8a For Efficient Cell Surface ExpressionThe Heterodimer Cd8a/Cd8b Localizes To Lipid Rafts Due To Cd8b Cytoplasmic Tail PalmitoylationIsoform 2: Cell MembraneIsoform 3: SecretedIsoform 4: Cell MembraneIsoform 5: Cell MembraneIsoform 6: SecretedIsoform 7: SecretedIsoform 8: Secreted |
| Alternative Peptide Names | T-Cell Surface Glycoprotein Cd8 Beta Chain proteinCd Antigen Cd8b proteinCD8B proteinCD8B1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
