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All you need to know when choosing a Gradient Gel Why use a gradient gel? If you’ve found yourself contemplating which type of polyacrylamide gel you wish to use for your western blotting protocol

Genetic strategies for antibody validation Researchers invest precious resources in studies that rely on the specificity of their chosen antibody. Often, the success or failure of a project hinges

Antibodies in immunofluorescence: top tips If you work with antibodies, the chances are that you’re familiar with immunofluorescence (IF), or at least the application term. This common laboratory t

Top tips for optimum SDS-PAGE results The process of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique in biochemistry, molecular biology and biotechno

FAQ Antibody Validation Project The Antibody Validation Project is a modern sampling platform. It gives you the opportunity to develop a complete picture of the products you need, and utilise the dat

Immunofluorescence Troubleshooting As most researchers know, getting a technique to work in the way it’s supposed to can be a matter of trial and error. Immunofluorescence (IF), a technique that us

The Bradford Protein Assay Tips - Preparing For Western Blotting The Bradford Protein Assay is a simple spectroscopic technique which is used in laboratory research to measure the total concentrati

How to calculate the Rf value of a protein from western blot Western blot is a common tool for identifying, quantifying and determining the size of specific proteins. Essentially, western blotting