BCKDH Positive Control peptide (STJ503711)
SPECIFICATIONS
General Information
| Short Description | BCKDH Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications | WB |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | WB: 1:500 |
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | BCKDK |
| Gene ID | 10295 |
| Uniprot ID | BCKD_HUMAN |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Autophosphorylated. |
| Function | Serine/threonine-protein kinase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle. Phosphorylates and inactivates mitochondrial BCKDH complex a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Associates with the E2 component of BCKDH complex and phosphorylates BCKDHA on Ser-337, leading to conformational changes that interrupt substrate channeling between E1 and E2 and inactivates the BCKDH complex. Phosphorylates ACLY on Ser-455 in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and glucogenesis, respectively. Recognizes phosphosites having SxxE/D canonical motif. |
| Peptide Name | Branched-Chain Alpha-Ketoacid Dehydrogenase KinaseBckdh KinaseBckdhkinBdk3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial |
| Database Links | Reactome: R-HSA-70895 |
| Cellular Localisation | Mitochondrion MatrixDetected In The Cytosolic Compartment Of Liver Cells |
| Alternative Peptide Names | Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase proteinBckdh Kinase proteinBckdhkin proteinBdk protein3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial proteinBCKDK protein |
Information sourced from Uniprot.org