• Immunofluorescence analysis of NIH-3T3 cells using Zyxin Rabbit monoclonal antibody (STJ11101808) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Zyxin Rabbit monoclonal antibody (STJ11101808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ZYX antibody (250-350) [S8MR] (STJ11101808)

SKU:
STJ11101808

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Zyxin (250-350) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZYX
Gene ID: 7791
Uniprot ID: ZYX_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Zyxin (Q15942).
Immunogen Sequence: TQPRGPPASSPAPAPKFSPV TPKFTPVASKFSPGAPGGSG SQPNQKLGHPEALSAGTGSP QPPSFTYAQQREKPRVQEKQ HPVPPPAQNQNQVRSPGAPG P
Function Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.
Protein Name Zyxin
Zyxin-2
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Cell Junction
Focal Adhesion
Associates With The Actin Cytoskeleton Near The Adhesion Plaques
Enters The Nucleus In The Presence Of Hesx1
Alternative Antibody Names Anti-Zyxin antibody
Anti-Zyxin-2 antibody
Anti-ZYX antibody

Information sourced from Uniprot.org

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