• Immunofluorescence analysis of NIH-3T3 cells using ZWINT Polyclonal Antibody (STJ28250) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ZWINT Polyclonal Antibody (STJ28250) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ZWINT Polyclonal Antibody (STJ28250) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ZWINT antibody (STJ28250) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ZWINT antibody (1-277) (STJ28250)

SKU:
STJ28250

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZWINT (1-277) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZWINT
Gene ID: 11130
Uniprot ID: ZWINT_HUMAN
Immunogen Region: 1-277
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human ZWINT (NP_008988.2).
Immunogen Sequence: MEAAETEAEAAALEVLAEVA GILEPVGLQEEAELPAKILV EFVVDSQKKDKLLCSQLQVA DFLQNILAQEDTAKGLDPLA SEDTSRQKAIAAKEQWKELK ATYREHVEAIKIGLTKALTQ MEEAQRKRTQLREAFEQLQA KKQMAMEKRRAVQNQWQLQQ EKHLQHLAEVSAEVRERKTG TQQELDRVFQKLGNLKQQAE QERDKLQRYQTFLQLLYTLQ GKLLFPEAEAEAENLPDDK
Function Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
Protein Name Zw10 Interactor
Zw10-Interacting Protein 1
Zwint-1
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Localizes To Kinetochores From Late Prophase To Anaphase
Alternative Antibody Names Anti-Zw10 Interactor antibody
Anti-Zw10-Interacting Protein 1 antibody
Anti-Zwint-1 antibody
Anti-ZWINT antibody

Information sourced from Uniprot.org

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