• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ZP2 antibody (Internal) (STJ96360)

SKU:
STJ96360

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Zona pellucida sperm-binding protein 2 (Internal) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZP2
Gene ID: 7783
Uniprot ID: ZP2_HUMAN
Immunogen Region: Internal
Specificity: ZP2 Polyclonal Antibody detects endogenous levels of ZP2 protein.
Immunogen: Synthesized peptide derived from the Internal region of human ZP2.
Post Translational Modifications Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. Proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos. N-glycosylated. O-glycosylated.contains sulfate-substituted glycans.
Function Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
Protein Name Zona Pellucida Sperm-Binding Protein 2
Zona Pellucida Glycoprotein 2
Zp-2
Zona Pellucida Protein A Cleaved Into - Processed Zona Pellucida Sperm-Binding Protein 2
Database Links Reactome: R-HSA-2534343
Cellular Localisation Processed Zona Pellucida Sperm-Binding Protein 2: Zona Pellucida
Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Zona Pellucida Sperm-Binding Protein 2 antibody
Anti-Zona Pellucida Glycoprotein 2 antibody
Anti-Zp-2 antibody
Anti-Zona Pellucida Protein A Cleaved Into - Processed Zona Pellucida Sperm-Binding Protein 2 antibody
Anti-ZP2 antibody
Anti-ZPA antibody

Information sourced from Uniprot.org

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