• Immunohistochemistry analysis of paraffin-embedded human liver cancer using ZNF259 Rabbit monoclonal antibody (STJ11102766) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from Mouse brain, using ZNF259 antibody (STJ11102766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ZNF259 antibody (360-459) [S6MR] (STJ11102766)

SKU:
STJ11102766

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ZNF259 (360-459) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZPR1
Gene ID: 8882
Uniprot ID: ZPR1_HUMAN
Immunogen Region: 360-459
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 360-459 of human ZNF259 (O75312).
Immunogen Sequence: DIRELVTKNPFTLGDSSNPG QTERLQEFSQKMDQIIEGNM KAHFIMDDPAGNSYLQNVYA PEDDPEMKVERYKRTFDQNE ELGLNDMKTEGYEAGLAPQR
Tissue Specificity Expressed in fibroblast.weakly expressed in fibroblast of spinal muscular atrophy (SMA) patients.
Function Acts as a signaling molecule that communicates proliferative growth signals from the cytoplasm to the nucleus. It is involved in the positive regulation of cell cycle progression. Plays a role for the localization and accumulation of the survival motor neuron protein SMN1 in sub-nuclear bodies, including gems and Cajal bodies. Induces neuron differentiation and stimulates axonal growth and formation of growth cone in spinal cord motor neurons. Plays a role in the splicing of cellular pre-mRNAs. May be involved in H(2)O(2)-induced neuronal cell death.
Protein Name Zinc Finger Protein Zpr1
Zinc Finger Protein 259
Cellular Localisation Nucleus
Nucleolus
Gem
Cajal Body
Cytoplasm
Perinuclear Region
Cell Projection
Axon
Growth Cone
Colocalized With Smn1 In Gemini Of Coiled Bodies (Gems)
Cajal Bodies
Axon And Growth Cones Of Neurons
Localized Predominantly In The Cytoplasm In Serum-Starved Cells Growth Arrested In G0 Of The Mitotic Cell Cycle
Localized Both In The Nucleus And Cytoplasm At The G1 Phase Of The Mitotic Cell Cycle
Accumulates In The Subnuclear Bodies During Progression Into The S Phase Of The Mitotic Cell Cycle
Diffusely Localized Throughout The Cell During Mitosis
Colocalized With Npat And Smn1 In Nuclear Bodies Including Gems (Gemini Of Coiled Bodies) And Cajal Bodies In A Cell Cycle-Dependent Manner
Translocates Together With Eef1a1 From The Cytoplasm To The Nucleolus After Treatment With Mitogens
Colocalized With Egfr In The Cytoplasm Of Quiescent Cells
Translocates From The Cytoplasm To The Nucleus In A Epidermal Growth Factor (Egf)-Dependent Manner
Alternative Antibody Names Anti-Zinc Finger Protein Zpr1 antibody
Anti-Zinc Finger Protein 259 antibody
Anti-ZPR1 antibody
Anti-ZNF259 antibody

Information sourced from Uniprot.org

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