• Western blot analysis of extracts of various cell lines, using ZIC1 Rabbit monoclonal antibody (STJ11103038) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ZIC1 antibody (1-100) [S8MR] (STJ11103038)

SKU:
STJ11103038

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ZIC1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZIC1
Gene ID: 7545
Uniprot ID: ZIC1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ZIC1 (Q15915).
Immunogen Sequence: MLLDAGPQYPAIGVTTFGAS RHHSAGDVAERDVGLGINPF ADGMGAFKLNPSSHELASAG QTAFTSQAPGYAAAAALGHH HHPGHVGSYSSAAFNSTRDF
Tissue Specificity CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.
Function Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.
Protein Name Zinc Finger Protein Zic 1
Zinc Finger Protein 201
Zinc Finger Protein Of The Cerebellum 1
Cellular Localisation Nucleus
Cytoplasm
Localizes In The Cytoplasm In Presence Of Mdfic Overexpression
Alternative Antibody Names Anti-Zinc Finger Protein Zic 1 antibody
Anti-Zinc Finger Protein 201 antibody
Anti-Zinc Finger Protein Of The Cerebellum 1 antibody
Anti-ZIC1 antibody
Anti-ZIC antibody
Anti-ZNF201 antibody

Information sourced from Uniprot.org

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