• Immunohistochemistry analysis of paraffin-embedded rat testis using PLZF Rabbit polyclonal antibody (STJ28426) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using PLZF Rabbit polyclonal antibody (STJ28426) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PLZF antibody (STJ28426) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ZBTB16 antibody (199-673) (STJ28426)

SKU:
STJ28426

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLZF (199-673) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZBTB16
Gene ID: 7704
Uniprot ID: ZBT16_HUMAN
Immunogen Region: 199-673
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 199-673 of human PLZF (NP_005997.2).
Immunogen Sequence: TKAAVDSLMTIGQSLLQGTL QPPAGPEEPTLAGGGRHPGV AEVKTEMMQVDEVPSQDSPG AAESSISGGMGDKVEERGKE GPGTPTRSSVITSARELHYG RE
Tissue Specificity Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.
Function Acts as a transcriptional repressor. Transcriptional repression may be mediated through recruitment of histone deacetylases to target promoters. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Protein Name Zinc Finger And Btb Domain-Containing Protein 16
Promyelocytic Leukemia Zinc Finger Protein
Zinc Finger Protein 145
Zinc Finger Protein Plzf
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Cellular Localisation Nucleus
Nuclear Body
Alternative Antibody Names Anti-Zinc Finger And Btb Domain-Containing Protein 16 antibody
Anti-Promyelocytic Leukemia Zinc Finger Protein antibody
Anti-Zinc Finger Protein 145 antibody
Anti-Zinc Finger Protein Plzf antibody
Anti-ZBTB16 antibody
Anti-PLZF antibody
Anti-ZNF145 antibody

Information sourced from Uniprot.org

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