• Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-YY1 antibody (230-310 aa) (STJ192391)

SKU:
STJ192391

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transcriptional repressor protein YY1 (230-310 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YY1
Gene ID: 7528
Uniprot ID: TYY1_HUMAN
Immunogen Region: 230-310 aa
Specificity: TYY1 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from the human protein at the amino acid range 230-310
Function Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'.some genes have been shown to contain a longer binding motif allowing enhanced binding.the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.proposed to target the INO80 complex to YY1-responsive elements.
Protein Name Transcriptional Repressor Protein Yy1
Delta Transcription Factor
Ino80 Complex Subunit S
Nf-E1
Yin And Yang 1
Yy-1
Database Links Reactome: R-HSA-5617472
Reactome: R-HSA-5689603
Reactome: R-HSA-5696394
Reactome: R-HSA-8866910
Reactome: R-HSA-9018519
Cellular Localisation Nucleus Matrix
Associated With The Nuclear Matrix
Alternative Antibody Names Anti-Transcriptional Repressor Protein Yy1 antibody
Anti-Delta Transcription Factor antibody
Anti-Ino80 Complex Subunit S antibody
Anti-Nf-E1 antibody
Anti-Yin And Yang 1 antibody
Anti-Yy-1 antibody
Anti-YY1 antibody
Anti-INO80S antibody

Information sourced from Uniprot.org

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