• Western blot analysis of extracts of various cell lines, using 14-3-3 gamma rabbit monoclonal antibody (STJ11102442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded mouse brain using 14-3-3 gamma rabbit monoclonal antibody (STJ11102442) at dilution of 1:100 (40x lens).

Anti-YWHAG antibody (14-3) [ARC1457] (STJ11102442)


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Host: Rabbit
Applications: WB, IHC
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal antibody anti-14-3-3 gamma (14-3) is suitable for use in Western Blot and Immunohistochemistry.
Clonality: Monoclonal
Clone ID: ARC1457
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: YWHAG
Gene ID: 7532
Uniprot ID: 1433G_HUMAN
Immunogen Region: 14-3
Immunogen: A synthesized peptide derived from human 14-3-3 gamma
Tissue Specificity Highly expressed in brain, skeletal muscle, and heart.
Post Translational Modifications Phosphorylated by various PKC isozymes.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Protein Name 14-3-3 Protein Gamma
Protein Kinase C Inhibitor Protein 1
Kcip-1 Cleaved Into - 14-3-3 Protein Gamma - N-Terminally Processed
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-75035
Reactome: R-HSA-8854518
Reactome: R-HSA-9614399
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-14-3-3 Protein Gamma antibody
Anti-Protein Kinase C Inhibitor Protein 1 antibody
Anti-Kcip-1 Cleaved Into - 14-3-3 Protein Gamma - N-Terminally Processed antibody
Anti-YWHAG antibody

Information sourced from Uniprot.org

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