• Western blot analysis of extracts of various cell lines, using YTHDC1 antibody (STJ29457) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-YTHDC1 antibody (1-74) (STJ29457)

SKU:
STJ29457

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-YTHDC1 (1-74) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YTHDC1
Gene ID: 91746
Uniprot ID: YTDC1_HUMAN
Immunogen Region: 1-74
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-74 of human YTHDC1 (NP_001026902.1).
Immunogen Sequence: MAADSREEKDGELNVLDDIL TEVPEQDDELYNPESEQDKN EKKGSKRKSDRMESTDTKRQ KPSVHSRQLVSKPL
Post Translational Modifications Tyrosine phosphorylated.
Function Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. May also regulate alternative splice site selection. Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs. Also recognizes and binds m6A on other RNA molecules. Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist. Also recognizes and binds m6A-containing single-stranded DNA. Involved in germline development: required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing.
Protein Name Yth Domain-Containing Protein 1
Splicing Factor Yt521
Yt521-B
Cellular Localisation Nucleus
Nucleus Speckle
Localizes To A Novel Subnuclear Structure
The Yt Bodies
Alternative Antibody Names Anti-Yth Domain-Containing Protein 1 antibody
Anti-Splicing Factor Yt521 antibody
Anti-Yt521-B antibody
Anti-YTHDC1 antibody
Anti-KIAA1966 antibody
Anti-YT521 antibody

Information sourced from Uniprot.org

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