Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g YARS antibody (STJ28874). Western blot was performed from the immunoprecipitate using YARS antibody (STJ28874) at a dilution of 1:1000.

Anti-YARS antibody (1-300) (STJ28874)

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STJ28874
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YARS1
Gene ID: 8565
Uniprot ID: SYYC_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human YARS (NP_003671.1).
Immunogen Sequence: MGDAPSPEEKLHLITRNLQE VLGEEKLKEILKERELKIYW GTATTGKPHVAYFVPMSKIA DFLKAGCEVTILFADLHAYL DNMKAPWELLELRVSYYENV IKAMLESIGVPLEKLKFIKG TDYQLSKEYTLDVYRLSSVV TQHDSKKAGAEVVKQVEHPL LSGLLYPGLQALDEEYLKVD AQFGGIDQRKIFTFAEKYLP ALGYSKRVHLMNPMVPGLTG SKMSSSEEESKIDLLDRKE
Function Tyrosine--tRNA ligase that catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) (Probable). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tyrosine--tRNA ligase activity. Activity is switched upon resveratrol-binding: resveratrol strongly inhibits the tyrosine--tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1.
Protein Name Tyrosine--Trna Ligase - Cytoplasmic
Tyrosyl-Trna Synthetase
Tyrrs Cleaved Into - Tyrosine--Trna Ligase - Cytoplasmic - N-Terminally Processed
Database Links Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic In Normal Conditions
Resveratrol-Binding In Response To Serum Starvation Promotes Relocalization To The Nucleus
Alternative Antibody Names Anti-Tyrosine--Trna Ligase - Cytoplasmic antibody
Anti-Tyrosyl-Trna Synthetase antibody
Anti-Tyrrs Cleaved Into - Tyrosine--Trna Ligase - Cytoplasmic - N-Terminally Processed antibody
Anti-YARS1 antibody
Anti-YARS antibody

Information sourced from Uniprot.org

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