• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using XRCC1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from Jurkat cells, using XRCC1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using XRCC1 Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-XRCC1 antibody (517-566 aa) (STJ96282)

SKU:
STJ96282

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA repair protein XRCC1 (517-566 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XRCC1
Gene ID: 7515
Uniprot ID: XRCC1_HUMAN
Immunogen Region: 517-566 aa
Specificity: XRCC1 Polyclonal Antibody detects endogenous levels of XRCC1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human XRCC1 at the amino acid range 517-566
Post Translational Modifications Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated.
Function Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity.
Protein Name Dna Repair Protein Xrcc1
X-Ray Repair Cross-Complementing Protein 1
Database Links Reactome: R-HSA-110381
Reactome: R-HSA-5649702
Reactome: R-HSA-5685939
Reactome: R-HSA-5696397
Reactome: R-HSA-6782210
Cellular Localisation Nucleus
Chromosome
Moves From The Nucleoli To The Global Nuclear Chromatin Upon Dna Damage
Recruited To Dna Damage Sites Fowwing Interaction With Poly-Adp-Ribose Chains
Alternative Antibody Names Anti-Dna Repair Protein Xrcc1 antibody
Anti-X-Ray Repair Cross-Complementing Protein 1 antibody
Anti-XRCC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance