• Immunohistochemistry analysis of XPD/ERCC2 in paraffin-embedded human colon carcinoma tissue using XPD/ERCC2 Rabbit monoclonal antibody (STJ11102973) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of XPD/ERCC2 in paraffin-embedded rat kidney tissue using XPD/ERCC2 Rabbit monoclonal antibody (STJ11102973) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of XPD/ERCC2 in paraffin-embedded mouse brain tissue using XPD/ERCC2 Rabbit monoclonal antibody (STJ11102973) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of XPD/ERCC2 in paraffin-embedded human colon tissue using XPD/ERCC2 Rabbit monoclonal antibody (STJ11102973) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of XPD/ERCC2 in paraffin-embedded human cervix cancer tissue using XPD/ERCC2 Rabbit monoclonal antibody (STJ11102973) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using XPD/ERCC2 Rabbit monoclonal antibody (STJ11102973) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-XPD/ERCC2 antibody (600-700) [S3MR] (STJ11102973)

SKU:
STJ11102973

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-XPD/ERCC2 (600-700) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERCC2
Gene ID: 2068
Uniprot ID: ERCC2_HUMAN
Immunogen Region: 600-700
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human XPD/ERCC2 (P18074).
Immunogen Sequence: ARGKVSEGIDFVHHYGRAVI MFGVPYVYTQSRILKARLEY LRDQFQIRENDFLTFDAMRH AAQCVGRAIRGKTDYGLMVF ADKRFARGDKRGKLPRWIQE H
Post Translational Modifications ISGylated.
Function ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.
Protein Name General Transcription And Dna Repair Factor Iih Helicase Subunit Xpd
Tfiih Subunit Xpd
Basic Transcription Factor 2 80 Kda Subunit
Btf2 P80
Cxpd
Dna Excision Repair Protein Ercc-2
Dna Repair Protein Complementing Xp-D Cells
Tfiih Basal Transcription Factor Complex 80 Kda Subunit
Tfiih 80 Kda Subunit
Tfiih P80
Xeroderma Pigmentosum Group D-Complementing Protein
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167246
Reactome: R-HSA-2564830
Reactome: R-HSA-427413
Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-674695
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6796648
Reactome: R-HSA-72086
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-73863
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-77075
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle
Alternative Antibody Names Anti-General Transcription And Dna Repair Factor Iih Helicase Subunit Xpd antibody
Anti-Tfiih Subunit Xpd antibody
Anti-Basic Transcription Factor 2 80 Kda Subunit antibody
Anti-Btf2 P80 antibody
Anti-Cxpd antibody
Anti-Dna Excision Repair Protein Ercc-2 antibody
Anti-Dna Repair Protein Complementing Xp-D Cells antibody
Anti-Tfiih Basal Transcription Factor Complex 80 Kda Subunit antibody
Anti-Tfiih 80 Kda Subunit antibody
Anti-Tfiih P80 antibody
Anti-Xeroderma Pigmentosum Group D-Complementing Protein antibody
Anti-ERCC2 antibody
Anti-XPD antibody
Anti-XPDC antibody

Information sourced from Uniprot.org

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