| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Protein Wnt-2 (231-330) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S5275RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:2000-1:8000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WNT2 |
| Gene ID: |
7472 |
| Uniprot ID: |
WNT2_HUMAN |
| Immunogen Region: |
231-330 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 231-330 of human WNT2 (NP_003382.1). |
| Immunogen Sequence: |
GDYLWRKYNGAIQVVMNQDG TGFTVANERFKKPTKNDLVY FENSPDYCIRDREAGSLGTA GRVCNLTSRGMDSCEVMCCG RGYDTSHVTRMTKCGCKFHW |
| Tissue Specificity | Expressed in brain in the thalamus, in fetal and adult lung and in placenta. |
| Post Translational Modifications | Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition. |
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Functions as upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons. |
| Protein Name | Protein Wnt-2Int-1-Like Protein 1Int-1-Related ProteinIrp |
| Database Links | Reactome: R-HSA-3238698Reactome: R-HSA-373080 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Protein Wnt-2 antibodyAnti-Int-1-Like Protein 1 antibodyAnti-Int-1-Related Protein antibodyAnti-Irp antibodyAnti-WNT2 antibodyAnti-INT1L1 antibodyAnti-IRP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Sonic Hedgehog (Sonic Hedgehog (Shh) ) knockout (KO) HeLa cells, using [KO Validated] Sonic Hedgehog (Shh) Rabbit polyclonal antibody (STJ119993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98778/382827/STJ119993_1__20955.1713151328.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Sonic Hedgehog (Sonic Hedgehog (Shh) ) knockout (KO) HeLa cells, using [KO Validated] Sonic Hedgehog (Shh) Rabbit polyclonal antibody (STJ119993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
