| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Serine/threonine-protein kinase WNK4 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WNK4 |
| Gene ID: |
65266 |
| Uniprot ID: |
WNK4_HUMAN |
| Specificity: |
WNK4 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from part of the human protein |
| Post Translational Modifications | Autophosphorylated at Ser-331 and Ser-335, promoting its activation. Phosphorylated by WNK1 and WNK3. Phosphorylated at Ser-575 in a MAP3K15/ASK3-dependent process in response to osmotic stress or hypotonic low-chloride stimulation. Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation. Also ubiquitinated by the BCR(KLHL2) complex. |
| Function | Serine/threonine-protein kinase component of the WNK4-SPAK/OSR1 kinase cascade, which acts as a key regulator of ion transport in the distal nephron and blood pressure. The WNK4-SPAK/OSR1 kinase cascade is composed of WNK4, which mediates phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK. Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity. Acts as a molecular switch that regulates the balance between renal salt reabsorption and K(+) secretion by modulating the activities of renal transporters and channels, including the Na-Cl cotransporter SLC12A3/NCC and the K(+) channel, KCNJ1/ROMK. Regulates NaCl reabsorption in the distal nephron by activating the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney: activates SLC12A3/NCC in a OXSR1/OSR1- and STK39/SPAK-dependent process. Also acts as a scaffold protein independently of its protein kinase activity: negatively regulates cell membrane localization of various transporters and channels (CFTR, KCNJ1/ROMK, SLC4A4, SLC26A9 and TRPV4) by clathrin-dependent endocytosis. Also inhibits the activity of the epithelial Na(+) channel (ENaC) SCNN1A, SCNN1B, SCNN1D in a inase-independent mechanism. May also phosphorylate NEDD4L. |
| Protein Name | Serine/Threonine-Protein Kinase Wnk4Protein Kinase Lysine-Deficient 4Protein Kinase With No Lysine 4 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Cell JunctionTight JunctionPresent Exclusively In Intercellular Junctions In The Distal Convoluted Tubule And In Both The Cytoplasm And Intercellular Junctions In The Cortical Collecting DuctWnk4 Is Part Of The Tight Junction Complex |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Wnk4 antibodyAnti-Protein Kinase Lysine-Deficient 4 antibodyAnti-Protein Kinase With No Lysine 4 antibodyAnti-WNK4 antibodyAnti-PRKWNK4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
