• Western blot analysis of extracts of various cell lines, using WIPI1 rabbit monoclonal antibody (STJ11102501) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using WIPI1 Rabbit monoclonal antibody (STJ11102501) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-WIPI1 antibody (1-100) [S1MR] (STJ11102501)

SKU:
STJ11102501

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-WIPI1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WIPI1
Gene ID: 55062
Uniprot ID: WIPI1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WIPI1 (Q5MNZ9).
Immunogen Sequence: MEAEAADAPPGGVESALSCF SFNQDCTSLATGTKAGYKLF SLSSVEQLDQVHGSNEIPDV YIVERLFSSSLVVVVSHTKP RQMNVYHFKKGTEICNYSYS
Tissue Specificity Ubiquitously expressed. Highly expressed in skeletal muscle, heart, testis, pancreas and placenta. Highly expressed in G361, Sk-mel-28, Sk-mel-13, WM852 and WM451 cells. Up-regulated in a variety of tumor tissues.
Function Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. Together with WDR45/WIPI4, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Also plays a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway.
Protein Name Wd Repeat Domain Phosphoinositide-Interacting Protein 1
Wipi-1
Atg18 Protein Homolog
Wd40 Repeat Protein Interacting With Phosphoinositides Of 49 Kda
Wipi 49 Kda
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-381038
Cellular Localisation Golgi Apparatus
Trans-Golgi Network
Endosome
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Preautophagosomal Structure Membrane
Peripheral Membrane Protein
Cytoplasm
Cytoskeleton
Trans Elements Of The Golgi And Peripheral Endosomes
Dynamically Cycles Through These Compartments And Is Susceptible To Conditions That Modulate Membrane Flux
Enriched In Clathrin-Coated Vesicles
Upon Starvation-Induced Autophagy
Accumulates At Subcellular Structures In The Cytoplasm: Enlarged Vesicular And Lasso-Like Structures
And Large Cup-Shaped Structures Predominantly Around The Nucleus
Recruitment To Autophagic Membranes Is Controlled By Mtmr14
Labile Microtubules Specifically Recruit Markers Of Autophagosome Formation Like Wipi1
Whereas Mature Autophagosomes May Bind To Stable Microtubules
Alternative Antibody Names Anti-Wd Repeat Domain Phosphoinositide-Interacting Protein 1 antibody
Anti-Wipi-1 antibody
Anti-Atg18 Protein Homolog antibody
Anti-Wd40 Repeat Protein Interacting With Phosphoinositides Of 49 Kda antibody
Anti-Wipi 49 Kda antibody
Anti-WIPI1 antibody
Anti-WIPI49 antibody

Information sourced from Uniprot.org

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