| Short Description : | Rabbit polyclonal WDR81 (340-1941) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | WDR81 |
| Gene ID: | 124997 |
| Uniprot ID: | WDR81_HUMAN |
| Immunogen Region: | 340-1941 |
| Immunogen Sequence: | TGQEELRSLVLDWVHGRISN FHYLMQLNRLAGRRQGDPNY HPVLPWVVDFTTPHGRFRDL RKSKFRLNKGDKQLDFTYEM TRQAFVAGGAGGGEPPHVPH HISDVLSDITYYVYKARRTP RSVLCGHVRAQWEPHEYPAS MERMQNWTPDECIPEFYTDP SIFRSIHPDMPDLDVPAWCS SSQEFVAAHRALLESREVSR DLHHWIDLTFGYKLQGKEAV KEKNVCLHLVDAHTHLASY |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-620 of human WDR81 (NP_001157281.1). |
| Tissue Specificity | Widely expressed. In the brain, highest levels in cerebellum and corpus callosum. |
| Function | Functions as a negative regulator of the PI3 kinase/PI3K activity associated with endosomal membranes via BECN1, a core subunit of the PI3K complex. By modifying the phosphatidylinositol 3-phosphate/PtdInsP3 content of endosomal membranes may regulate endosome fusion, recycling, sorting and early to late endosome transport. It is for instance, required for the delivery of cargos like BST2/tetherin from early to late endosome and thereby participates indirectly to their degradation by the lysosome. May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated protein aggregates. In this process, may regulate the interaction of SQSTM1 with ubiquitinated proteins and also recruit MAP1LC3C. May also be involved in maintenance of normal mitochondrial structure and organization. |
| Protein Name | Wd Repeat-Containing Protein 81 |
| Database Links | Reactome: R-HSA-9013148 |
| Cellular Localisation | Early Endosome MembranePeripheral Membrane ProteinLate Endosome MembraneLysosome MembraneCytoplasmic VesicleAutophagosome MembraneMitochondrionCytoplasmCytosol |
| Alternative Antibody Names | Anti-Wd Repeat-Containing Protein 81 antibodyAnti-WDR81 antibody |
Information sourced from Uniprot.org
