• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using WDR4 Rabbit monoclonal antibody (STJ11103120) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using WDR4 Rabbit monoclonal antibody (STJ11103120) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using WDR4 Rabbit monoclonal antibody (STJ11103120) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WDR4 antibody (250-350) [S0MR] (STJ11103120)

SKU:
STJ11103120

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-WDR4 (250-350) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WDR4
Gene ID: 10785
Uniprot ID: WDR4_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human WDR4 (P57081).
Immunogen Sequence: AFWCQENCVALLCDGTPVVY IFQLDARRQQLVYRQQLAFQ HQVWDVAFEETQGLWVLQDC QEAPLVLYRPVGDQWQSVPE STVLKKVSGVLRGNWAMLEG S
Function Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs. Also required for methylation of a specific subset of miRNAs, such as let-7. Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1.
Protein Name Trna
Guanine-N(7--Methyltransferase Non-Catalytic Subunit Wdr4
Protein Wuho Homolog
Hwh
Wd Repeat-Containing Protein 4
Database Links Reactome: R-HSA-6782315
Cellular Localisation Nucleus
Chromosome
Localizes At The Site Of Nascent Dna Synthesis
Alternative Antibody Names Anti-Trna antibody
Anti-Guanine-N(7--Methyltransferase Non-Catalytic Subunit Wdr4 antibody
Anti-Protein Wuho Homolog antibody
Anti-Hwh antibody
Anti-Wd Repeat-Containing Protein 4 antibody
Anti-WDR4 antibody

Information sourced from Uniprot.org

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