Anti-WDR4 antibody (250-350) [S0MR] (STJ11103120)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal WDR4 (250-350) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S0MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IHC-P:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WDR4 |
| Gene ID | 10785 |
| Uniprot ID | WDR4_HUMAN |
| Immunogen Region | 250-350 |
| Immunogen Sequence | AFWCQENCVALLCDGTPVVY IFQLDARRQQLVYRQQLAFQ HQVWDVAFEETQGLWVLQDC QEAPLVLYRPVGDQWQSVPE STVLKKVSGVLRGNWAMLEG S |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human WDR4 (P57081). |
Additional Info
| Function | Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs. Also required for methylation of a specific subset of miRNAs, such as let-7. Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1. |
| Protein Name | TrnaGuanine-N(7--Methyltransferase Non-Catalytic Subunit Wdr4Protein Wuho HomologHwhWd Repeat-Containing Protein 4 |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | NucleusChromosomeLocalizes At The Site Of Nascent Dna Synthesis |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Guanine-N(7--Methyltransferase Non-Catalytic Subunit Wdr4 antibodyAnti-Protein Wuho Homolog antibodyAnti-Hwh antibodyAnti-Wd Repeat-Containing Protein 4 antibodyAnti-WDR4 antibody |
Information sourced from Uniprot.org