| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S0MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:6000 IHC-P:1:100-1:500 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | WDR4 |
| Gene ID: | 10785 |
| Uniprot ID: | WDR4_HUMAN |
| Immunogen Region: | 250-350 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human WDR4 (P57081). |
| Immunogen Sequence: | AFWCQENCVALLCDGTPVVY IFQLDARRQQLVYRQQLAFQ HQVWDVAFEETQGLWVLQDC QEAPLVLYRPVGDQWQSVPE STVLKKVSGVLRGNWAMLEG S |
| Function | Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs. Also required for methylation of a specific subset of miRNAs, such as let-7. Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1. |
| Protein Name | Trna Guanine-N(7--Methyltransferase Non-Catalytic Subunit Wdr4 Protein Wuho Homolog Hwh Wd Repeat-Containing Protein 4 |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | Nucleus Chromosome Localizes At The Site Of Nascent Dna Synthesis |
| Alternative Antibody Names | Anti-Trna antibody Anti-Guanine-N(7--Methyltransferase Non-Catalytic Subunit Wdr4 antibody Anti-Protein Wuho Homolog antibody Anti-Hwh antibody Anti-Wd Repeat-Containing Protein 4 antibody Anti-WDR4 antibody |
Information sourced from Uniprot.org