Anti-VRK2 antibody (171-220 aa) (STJ96257)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human VRK2 at the amino acid range 171-220
General Information
| Short Description | Rabbit polyclonal anti-Serine/threonine-protein kinase VRK2 (171-220 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VRK2 |
| Gene ID | 7444 |
| Uniprot ID | VRK2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human VRK2 at the amino acid range 171-220 |
| Immunogen Region | 171-220 aa |
| Specificity | VRK2 Polyclonal Antibody detects endogenous levels of VRK2 protein. |
Additional Info
| Post Translational Modifications | Isoform 1: Autophosphorylated. Isoform 2: Autophosphorylated. |
| Function | Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Down-regulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant. Isoform 2: Phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity. |
| Protein Name | Serine/Threonine-Protein Kinase Vrk2Vaccinia-Related Kinase 2 |
| Database Links | Reactome: R-HSA-2980766 Q86Y07-2Reactome: R-HSA-2995383 Q86Y07-2Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013408Reactome: R-HSA-9013423 |
| Cellular Localisation | Isoform 1: CytoplasmEndoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinMitochondrion MembraneNucleus EnvelopeIsoform 2: CytoplasmNucleus |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Vrk2 antibodyAnti-Vaccinia-Related Kinase 2 antibodyAnti-VRK2 antibody |
Information sourced from Uniprot.org