• Western blot analysis of lysates from wild type (WT) and VRK1 knockout (KO) 293T cells, using [KO Validated] VRK1 Rabbit polyclonal antibody (STJ11100814) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-VRK1 antibody (257-396) (STJ11100814)

SKU:
STJ11100814

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VRK1 (257-396) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VRK1
Gene ID: 7443
Uniprot ID: VRK1_HUMAN
Immunogen Region: 257-396
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1).
Immunogen Sequence: GHLPWEDNLKDPKYVRDSKI RYRENIASLMDKCFPEKNKP GEIAKYMETVKLLDYTEKPL YENLRDILLQGLKAIGSKDD GKLDLSVVENGGLKAKTITK KRKKEIEESKEPGVEDTEWS NTQTEEAIQTRSRTRKRVQK
Tissue Specificity Widely expressed. Highly expressed in fetal liver, testis and thymus.
Post Translational Modifications Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis.
Function Serine/threonine kinase involved in cell cycle, nuclear condensation and transcription regulation. Involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.
Protein Name Serine/Threonine-Protein Kinase Vrk1
Vaccinia-Related Kinase 1
Database Links Reactome: R-HSA-2980766
Reactome: R-HSA-2995383
Cellular Localisation Nucleus
Cytoplasm
Dispersed Throughout The Cell But Not Located On Mitotic Spindle Or Chromatids During Mitosis
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Vrk1 antibody
Anti-Vaccinia-Related Kinase 1 antibody
Anti-VRK1 antibody

Information sourced from Uniprot.org

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