Anti-VPS18 antibody (1-100) [S5MR] (STJ11102915)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal VPS18 (1-100) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VPS18 |
| Gene ID | 57617 |
| Uniprot ID | VPS18_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MASILDEYENSLSRSAVLQP GCPSVGIPHSGYVNAQLEKE VPIFTKQRIDFTPSERITSL VVSSNQLCMSLGKDTLLRID LGKANEPNHVELGRKDDAKV |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS18 (Q9P253). |
Additional Info
| Tissue Specificity | Ubiquitous. Expression was highest in heart and low in lung. |
| Function | Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes. Involved in dendrite development of Pukinje cells. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 18 HomologHvps18 |
| Database Links | Reactome: R-HSA-9754560 |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinCytoplasmic SideLysosome MembraneEarly EndosomeCytoplasmic VesicleAutophagosomeClathrin-Coated VesicleCytoplasmicPeripheral Membrane Protein Associated With Early Endosomes And Late Endosomes/Lysosomes |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 18 Homolog antibodyAnti-Hvps18 antibodyAnti-VPS18 antibodyAnti-KIAA1475 antibody |
Information sourced from Uniprot.org