| Host: |
NZ White Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Nz White Rabbit polyclonal antibody anti-VGluT2 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Shipped as lyophilised. Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material. |
| Purification: |
Ammonium sulphate precipitation |
| Dilution Range: |
A concentration of 10-50 ug/ml recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Slc17a6 |
| Gene ID: |
84487 |
| Uniprot ID: |
VGLU2_RAT |
| Specificity: |
Specific for VGLUT2. |
| Immunogen: |
A synthetic peptide from mouse VGluT2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat. |
| Function | Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as a uniporter which transports preferentially L-glutamate but also, phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane therefore affects the proton electrochemical gradient and promotes synaptic vesicles acidification. Moreover, functions as a vesicular K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular L-glutamate uptake. The vesicular H(+)/H(+) antiport activity is electroneutral. At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation. The symporter activity is driven by an inside negative membrane potential and is electrogenic. Also involved in the regulation of retinal hyaloid vessel regression during postnatal development. May also play a role in the endocrine L-glutamatergic system of other tissues such as pineal gland and pancreas. |
| Protein Name | Vesicular Glutamate Transporter 2Vglut2Differentiation-Associated BnpiDifferentiation-Associated Na(+-Dependent Inorganic Phosphate CotransporterSolute Carrier Family 17 Member 6 |
| Database Links | Reactome: R-RNO-428643 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneMulti-Pass Membrane ProteinSynapseSynaptosomeCell Membrane |
| Alternative Antibody Names | Anti-Vesicular Glutamate Transporter 2 antibodyAnti-Vglut2 antibodyAnti-Differentiation-Associated Bnpi antibodyAnti-Differentiation-Associated Na(+-Dependent Inorganic Phosphate Cotransporter antibodyAnti-Solute Carrier Family 17 Member 6 antibodyAnti-Slc17a6 antibodyAnti-Dnpi antibodyAnti-Vglut2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
