• Immunohistochemistry analysis of paraffin-embedded Rat testis using Vgll4 Rabbit polyclonal antibody (STJ11100205) at dilution of 1:100 (20x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat testis using Vgll4 Rabbit polyclonal antibody (STJ11100205) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Mouse brain using Vgll4 Rabbit polyclonal antibody (STJ11100205) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of OVCAR3 cells, using Vgll4 antibody (STJ11100205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-VGLL4 antibody (1-287) (STJ11100205)

SKU:
STJ11100205

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Vgll4 (1-287) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Vgll4
Gene ID: 232334
Uniprot ID: VGLL4_MOUSE
Immunogen Region: 1-287
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of mouse Vgll4 (NP_808351.1).
Immunogen Sequence: MLFMKMDLLNYQYLDKMNNN IGVLCYEGEASLRGEPRMQT LPVASALSSHRTGPPPISPS KRKFSMEPGDKDLDCENDHV SKMSRIFSPHLNKTVNGDCR RDPRERSRSPIERAAAPAVS LHGGHLYASLPSLMEQPLAL TKNSSDTGRSAVERQQNRPS VITCASAGARNCNLSHCPIA HSGCSAPGSASYRRPPSATA TCDPVVEEHFRRSLGKNYKE PEPAPNSVSITGSVDDHFA
Function May act as a specific coactivator for the mammalian TEFs.
Protein Name Transcription Cofactor Vestigial-Like Protein 4
Vgl-4
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Cofactor Vestigial-Like Protein 4 antibody
Anti-Vgl-4 antibody
Anti-Vgll4 antibody

Information sourced from Uniprot.org

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