• Western blot analysis of extracts of Rat thymus, using VAV1 antibody (STJ117302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using VAV1 antibody (STJ117302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VAV1 antibody (374-590) (STJ117302)

SKU:
STJ117302

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VAV1 (374-590) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAV1
Gene ID: 7409
Uniprot ID: VAV_HUMAN
Immunogen Region: 374-590
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 374-590 of human VAV1 (NP_005419.2).
Immunogen Sequence: KRDNETLRQITNFQLSIENL DQSLAHYGRPKIDGELKITS VERRSKMDRYAFLLDKALLI CKRRGDSYDLKDFVNLHSFQ VRDDSSGDRDNKKWSHMFLL IEDQGAQGYELFFKTRELKK KWMEQFEMAISNIYPENATA NGHDFQMFSFEETTSCKACQ MLLRGTFYQGYRCHRCRASA HKECLGRVPPCGRHGQDFPG TMKKDKLHRRAQDKKRN
Tissue Specificity Widely expressed in hematopoietic cells but not in other cell types.
Post Translational Modifications Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS). Phosphorylated by FYN.
Function Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Protein Name Proto-Oncogene Vav
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1257604
Reactome: R-HSA-1433557
Reactome: R-HSA-193648
Reactome: R-HSA-2029482
Reactome: R-HSA-2219530
Reactome: R-HSA-2871796
Reactome: R-HSA-2871809
Reactome: R-HSA-389359
Reactome: R-HSA-416482
Reactome: R-HSA-4420097
Reactome: R-HSA-512988
Reactome: R-HSA-5218920
Reactome: R-HSA-6811558
Reactome: R-HSA-8980692
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013408
Reactome: R-HSA-9027284
Reactome: R-HSA-912631
Reactome: R-HSA-9664422
Reactome: R-HSA-9679191
Reactome: R-HSA-9748787
Reactome: R-HSA-983695
Alternative Antibody Names Anti-Proto-Oncogene Vav antibody
Anti-VAV1 antibody
Anti-VAV antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance