• Immunofluorescence analysis of L929 cells using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and VAT1 knockout (KO) 293T cells, using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-VAT1 antibody (200-300) (STJ119297)

SKU:
STJ119297

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VAT1 (200-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAT1
Gene ID: 10493
Uniprot ID: VAT1_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human VAT1 (NP_006364.2).
Immunogen Sequence: GVGMAAVQLCRTVENVTVFG TASASKHEALKENGVTHPID YHTTDYVDEIKKISPKGVDI VMDPLGGSDTAKGYNLLKPM GKVVTYGMANLLTGPKRNLM A
Tissue Specificity Expressed in brain. Also expressed in glioblastoma cells.
Function Possesses ATPase activity. Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).
Protein Name Synaptic Vesicle Membrane Protein Vat-1 Homolog
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Mitochondrion Outer Membrane
Peripheral Membrane Protein
The Majority Is Localized In The Cytoplasm And A Small Amount Is Associated With Mitochondria
Alternative Antibody Names Anti-Synaptic Vesicle Membrane Protein Vat-1 Homolog antibody
Anti-VAT1 antibody

Information sourced from Uniprot.org

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