• Immunohistochemistry analysis of paraffin-embedded human appendix using VAMP8 Rabbit monoclonal antibody (STJ11103576) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using VAMP8 antibody (STJ11103576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using VAMP8 antibody (STJ11103576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VAMP8 antibody (1-100) [S6MR] (STJ11103576)

SKU:
STJ11103576

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-VAMP8 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAMP8
Gene ID: 8673
Uniprot ID: VAMP8_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VAMP8 (NP_003752.2).
Immunogen Sequence: MEEASEGGGNDRVRNLQSEV EGVKNIMTQNVERILARGEN LEHLRNKTEDLEATSEHFKT TSQKVARKFWWKNVKMIVLI CVIVFIIILFIVLFATGAFS
Tissue Specificity Platelets.
Post Translational Modifications (Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton.
Function SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Also plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism.
Protein Name Vesicle-Associated Membrane Protein 8
Vamp-8
Endobrevin
Edb
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-199992
Reactome: R-HSA-432720
Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Lysosome Membrane
Single-Pass Type Iv Membrane Protein
Early Endosome Membrane
Late Endosome Membrane
Cell Membrane
Zymogen Granule Membrane
Perinuclear Vesicular Structures Of The Early And Late Endosomes
Coated Pits
And Trans-Golgi
Sub-Tight Junctional Domain In Retinal Pigment Epithelium Cells
Midbody Region During Cytokinesis
Lumenal Oriented
Apical Membranes Of Nephric Tubular Cell
Cycles Through The Apical But Not Through The Basolateral Plasma Membrane
Apical Region Of Acinar Cells
In Zymogen Granule Membranes
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein 8 antibody
Anti-Vamp-8 antibody
Anti-Endobrevin antibody
Anti-Edb antibody
Anti-VAMP8 antibody

Information sourced from Uniprot.org

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