• Western blot analysis of extracts of various cell lines, using VAMP3/VAMP3/Cellubrevin rabbit monoclonal antibody (STJ11102379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using VAMP3/VAMP3/Cellubrevin Rabbit monoclonal antibody (STJ11102379) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of mouse liver, using VAMP3/VAMP3/Cellubrevin rabbit monoclonal antibody (STJ11102379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from Mouse liver, using VAMP3/VAMP3/Cellubrevin Rabbit monoclonal antibody (STJ11102379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of U-2 OS cells using VAMP3/VAMP3/Cellubrevin rabbit monoclonal antibody (STJ11102379) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using VAMP3/VAMP3/Cellubrevin Rabbit monoclonal antibody (STJ11102379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-VAMP3 antibody (1-100) [S9MR] (STJ11102379)

SKU:
STJ11102379

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cellubrevin (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAMP3
Gene ID: 9341
Uniprot ID: VAMP3_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human VAMP3/Cellubrevin (Q15836).
Immunogen Sequence: MSTGPTAATGSNRRLQQTQN QVDEVVDIMRVNVDKVLERD QKLSELDDRADALQAGASQF ETSAAKLKRKYWWKNCKMWA IGITVLVIFIIIIIVWVVSS
Post Translational Modifications Ubiquitinated by RNF167 at Lys-66, Lys-68 and Lys-77, regulating the recycling endosome pathway. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which hydrolyzes the 59-Gln-|-Phe-60 bond and probably inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which hydrolyzes the 42-Lys-|-Leu-43 bond and probably inhibits neurotransmitter release. Note that humans are not known to be infected by C.botulinum type D. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 41-Gln-|-Lys-42 bond and probably inhibits neurotransmitter release.
Function SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
Protein Name Vesicle-Associated Membrane Protein 3
Vamp-3
Cellubrevin
Ceb
Synaptobrevin-3
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-6811440
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Reactome: R-HSA-9035034
Cellular Localisation Early Endosome Membrane
Single-Pass Type Iv Membrane Protein
Recycling Endosome Membrane
Single-Pass Type I Membrane Protein
Synapse
Synaptosome
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein 3 antibody
Anti-Vamp-3 antibody
Anti-Cellubrevin antibody
Anti-Ceb antibody
Anti-Synaptobrevin-3 antibody
Anti-VAMP3 antibody
Anti-SYB3 antibody

Information sourced from Uniprot.org

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