| Short Description : | Rabbit polyclonal Inactive Ubiquitin Carboxyl-Terminal Hydrolase 53 (495-582) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | USP53 |
| Gene ID: | 54532 |
| Uniprot ID: | UBP53_HUMAN |
| Immunogen Region: | 495-582 |
| Immunogen Sequence: | APNGFKQHGNPHLYHSQGKG SYKHDRVVPQSRASAQIISS SKSQILAPGEKITGKVKSDN GTGYDTDSSQDSRDRGNSCD SSSKSRNR |
| Specificity: | Recombinant Protein corresponding to a sequence within amino acids 495-582 of human USP53 (NP_061923.2). |
| Function | Deubiquitinase that mediates 'Lys-63'-linked deubiquitination of tight junction proteins, such as MARVELD2 and LSR, and which is involved in the survival of auditory hair cells and hearing. Specifically cleaves 'Lys-63'-linked polyubiquitin chains composed of at least 3 ubiquitin molecules, while it is not able to deubiquitinate substrates with shorter ubiquitin chains: recognizes ubiquitin chain in position S2 and catalyzes en bloc cleavage of polyubiquitin chains from substrate proteins. Probably acts by modulating the barrier properties and mechanical stability of tight junctions via deubiquitination of MARVELD2 and LSR. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 53Ubiquitin-Specific Peptidase 53 |
| Database Links | |
| Cellular Localisation | Cell JunctionTight Junction |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 53 antibodyAnti-Ubiquitin-Specific Peptidase 53 antibodyAnti-USP53 antibodyAnti-KIAA1350 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) HeLa (KO) cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ114797) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/93884/375089/STJ114797_1__72218.1713141770.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) HeLa (KO) cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ114797) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
