• Immunofluorescence analysis of Hep G2 cells using USP53 Rabbit polyclonal antibody (STJ11105514) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded Human liver cancer using USP53 Rabbit polyclonal antibody (STJ11105514) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of lysates from Mouse placenta using USP53 Rabbit polyclonal antibody (STJ11105514) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:10s.

Anti-USP53 antibody (495-582) (STJ11105514)

SKU:
STJ11105514

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Inactive Ubiquitin Carboxyl-Terminal Hydrolase 53 (495-582) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP53
Gene ID: 54532
Uniprot ID: UBP53_HUMAN
Immunogen Region: 495-582
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 495-582 of human USP53 (NP_061923.2).
Immunogen Sequence: APNGFKQHGNPHLYHSQGKG SYKHDRVVPQSRASAQIISS SKSQILAPGEKITGKVKSDN GTGYDTDSSQDSRDRGNSCD SSSKSRNR
Function Tight junction-associated protein that is involved in the survival of auditory hair cells and hearing. Maybe by modulating the barrier properties and mechanical stability of tight junctions. Has no peptidase activity.
Protein Name Inactive Ubiquitin Carboxyl-Terminal Hydrolase 53
Inactive Ubiquitin-Specific Peptidase 53
Cellular Localisation Cell Junction
Tight Junction
Alternative Antibody Names Anti-Inactive Ubiquitin Carboxyl-Terminal Hydrolase 53 antibody
Anti-Inactive Ubiquitin-Specific Peptidase 53 antibody
Anti-USP53 antibody
Anti-KIAA1350 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance