| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | USP53 |
| Gene ID: | 54532 |
| Uniprot ID: | UBP53_HUMAN |
| Immunogen Region: | 495-582 |
| Specificity: | Recombinant Protein corresponding to a sequence within amino acids 495-582 of human USP53 (NP_061923.2). |
| Immunogen Sequence: | APNGFKQHGNPHLYHSQGKG SYKHDRVVPQSRASAQIISS SKSQILAPGEKITGKVKSDN GTGYDTDSSQDSRDRGNSCD SSSKSRNR |
| Function | Tight junction-associated protein that is involved in the survival of auditory hair cells and hearing. Maybe by modulating the barrier properties and mechanical stability of tight junctions. Has no peptidase activity. |
| Protein Name | Inactive Ubiquitin Carboxyl-Terminal Hydrolase 53Inactive Ubiquitin-Specific Peptidase 53 |
| Cellular Localisation | Cell JunctionTight Junction |
| Alternative Antibody Names | Anti-Inactive Ubiquitin Carboxyl-Terminal Hydrolase 53 antibodyAnti-Inactive Ubiquitin-Specific Peptidase 53 antibodyAnti-USP53 antibodyAnti-KIAA1350 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) 293T cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ11100746) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89684/358175/STJ11100746_1__66574.1713122483.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) 293T cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ11100746) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.")
