• Immunofluorescence analysis of L-929 cells using USP46 Rabbit polyclonal antibody (STJ119875) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using USP46 Rabbit polyclonal antibody (STJ119875) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from mouse pancreas, using USP46 Rabbit polyclonal antibody (STJ119875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-USP46 antibody (100-200) (STJ119875)

SKU:
STJ119875

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP46 (100-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP46
Gene ID: 64854
Uniprot ID: UBP46_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human USP46 (NP_073743.2).
Immunogen Sequence: KKFISRLRKENDLFDNYMQQ DAHEFLNYLLNTIADILQEE KKQEKQNGKLKNGNMNEPAE NNKPELTWVHEIFQGTLTNE TRCLNCETVSSKDEDFLDLS V
Tissue Specificity Broadly expressed.
Function Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 46
Deubiquitinating Enzyme 46
Ubiquitin Thioesterase 46
Ubiquitin-Specific-Processing Protease 46
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 46 antibody
Anti-Deubiquitinating Enzyme 46 antibody
Anti-Ubiquitin Thioesterase 46 antibody
Anti-Ubiquitin-Specific-Processing Protease 46 antibody
Anti-USP46 antibody

Information sourced from Uniprot.org

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