• Western blot analysis of extracts of various cell lines, using USP22 rabbit monoclonal antibody (STJ11102464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from Rat lung, using USP22 Rabbit monoclonal antibody (STJ11102464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of mouse thymus, using USP22 rabbit monoclonal antibody (STJ11102464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from Mouse thymus, using USP22 Rabbit monoclonal antibody (STJ11102464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of Rat lung, using USP22 rabbit monoclonal antibody (STJ11102464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of lysates from HepG2 cells, using USP22 Rabbit monoclonal antibody (STJ11102464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-USP22 antibody (1-175) [S4MR] (STJ11102464)

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STJ11102464

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-USP22 (1-175) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP22
Gene ID: 23326
Uniprot ID: UBP22_HUMAN
Immunogen Region: 1-175
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human USP22 (Q9UPT9).
Immunogen Sequence: MVSRPEPEGEAMDAELAVAP PGCSHLGSFKVDNWKQNLRA IYQCFVWSGTAEARKRKAKS CICHVCGVHLNRLHSCLYCV FFGCFTKKHIHEHAKAKRHN LAIDLMYGGIYCFLCQDYIY DKDMEIIAKEEQRKAWKMQG VGEKFSTWEPTKRELELLKH NPKRRKITSNCTIGL
Tissue Specificity Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
Function Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 22
Deubiquitinating Enzyme 22
Ubiquitin Thioesterase 22
Ubiquitin-Specific-Processing Protease 22
Database Links Reactome: R-HSA-3214847
Reactome: R-HSA-5689880
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 22 antibody
Anti-Deubiquitinating Enzyme 22 antibody
Anti-Ubiquitin Thioesterase 22 antibody
Anti-Ubiquitin-Specific-Processing Protease 22 antibody
Anti-USP22 antibody
Anti-KIAA1063 antibody
Anti-USP3L antibody

Information sourced from Uniprot.org

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