Anti-USP1 antibody (1-280) (STJ28868)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal USP1 (1-280) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP1 |
| Gene ID | 7398 |
| Uniprot ID | UBP1_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MPGVIPSESNGLSRGSPSKK NRLSLKFFQKKETKRALDFT DSQENEEKASEYRASEIDQV VPAAQSSPINCEKRENLLPF VGLNNLGNTCYLNSILQVLY FCPGFKSGVKHLFNIISRKK EALKDEANQKDKGNCKEDSL ASYELICSLQSLIISVEQLQ ASFLLNPEKYTDELATQPRR LLNTLRELNPMYEGYLQHDA QEVLQCILGNIQETCQLLKK EEVKNVAELPTKVEEIPHP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP1 (NP_003359.3). |
Additional Info
| Post Translational Modifications | Autocatalytic cleavage of USP1 following UV irradiation inactivates it, leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis. Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment: Ubiquitinated by the CRL2(KLHDC2) complex following autocatalytic cleavage, leading to its degradation: the CRL2(KLHDC2) complex recognizes the diglycine (Gly-Gly) at the C-terminus. |
| Function | Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 1Deubiquitinating Enzyme 1HubpUbiquitin Thioesterase 1Ubiquitin-Specific-Processing Protease 1 Cleaved Into - Ubiquitin Carboxyl-Terminal Hydrolase 1 - N-Terminal Fragment |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-6783310 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 1 antibodyAnti-Deubiquitinating Enzyme 1 antibodyAnti-Hubp antibodyAnti-Ubiquitin Thioesterase 1 antibodyAnti-Ubiquitin-Specific-Processing Protease 1 Cleaved Into - Ubiquitin Carboxyl-Terminal Hydrolase 1 - N-Terminal Fragment antibodyAnti-USP1 antibody |
Information sourced from Uniprot.org