• Western blot analysis of various lysates, using UCHL5 antibody (STJ110285) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using UCHL5 rabbit polyclonal antibody (STJ110285) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using UCHL5 rabbit polyclonal antibody (STJ110285) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using UCHL5 rabbit polyclonal antibody (STJ110285) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using UCHL5 rabbit polyclonal antibody (STJ110285) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-UCHL5 antibody (1-328) (STJ110285)

SKU:
STJ110285

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UCHL5 (1-328) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UCHL5
Gene ID: 51377
Uniprot ID: UCHL5_HUMAN
Immunogen Region: 1-328
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human UCHL5 (NP_001186190.1).
Immunogen Sequence: MTGNAGEWCLMESDPGVFTE LIKGFGCRGAQVEEIWSLEP ENFEKLKPVHGLIFLFKWQP GEEPAGSVVQDSRLDTIFFA KQVINNACATQAIVSVLLNC THQDVHLGETLSEFKEFSQS FDAAMKGLALSNSDVIRQVH NSFARQQMFEFDTKTSAKEE DAFHFVSYVPVNGRLYELDG LREGPIDLGACNQDDWISAV RPVIEKRIQKYSEGEIRFNL MAIVSDRKMIYEQKIAELQ
Function Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex.however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L5
Uch-L5
Ubiquitin C-Terminal Hydrolase Uch37
Ubiquitin Thioesterase L5
Database Links Reactome: R-HSA-2173788
Reactome: R-HSA-5689603
Cellular Localisation Cytoplasm
Nucleus
Associates With The Proteasome 19s Subunit In The Cytoplasm
Associates With The Ino80 Complex In The Nucleus
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L5 antibody
Anti-Uch-L5 antibody
Anti-Ubiquitin C-Terminal Hydrolase Uch37 antibody
Anti-Ubiquitin Thioesterase L5 antibody
Anti-UCHL5 antibody
Anti-UCH37 antibody
Anti-AD-019 antibody
Anti-CGI-70 antibody

Information sourced from Uniprot.org

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