| Short Description : | Rabbit polyclonal anti-E3 ubiquitin-protein ligase UBR1 (821-870 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC/IF/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBR1 |
| Gene ID: | 197131 |
| Uniprot ID: | UBR1_HUMAN |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human UBR1 at the amino acid range 821-870 |
| Immunogen Region: | 821-870 aa |
| Specificity: | Ubr1 Polyclonal Antibody detects endogenous levels of Ubr1 protein. |
| Function | E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Recognizes both type-1 and type-2 N-degrons, containing positively charged amino acids (Arg, Lys and His) and bulky and hydrophobic amino acids, respectively. Does not ubiquitinate proteins that are acetylated at the N-terminus. In contrast, it strongly binds methylated N-degrons. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. |
| Protein Name | E3 Ubiquitin-Protein Ligase Ubr1N-Recognin-1Ubiquitin-Protein Ligase E3-Alpha-1Ubiquitin-Protein Ligase E3-Alpha-I |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Ubr1 antibodyAnti-N-Recognin-1 antibodyAnti-Ubiquitin-Protein Ligase E3-Alpha-1 antibodyAnti-Ubiquitin-Protein Ligase E3-Alpha-I antibodyAnti-UBR1 antibody |
Information sourced from Uniprot.org
