| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBE2D4 |
| Gene ID: | 51619 |
| Uniprot ID: | UB2D4_HUMAN |
| Immunogen Region: | 1-147 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D4 (NP_057067.1). |
| Immunogen Sequence: | MALKRIQKELTDLQRDPPAQ CSAGPVGDDLFHWQATIMGP NDSPYQGGVFFLTIHFPTDY PFKPPKVAFTTKIYHPNINS NGSICLDILRSQWSPALTVS KVLLSICSLLCDPNPDDPLV PEIAHTYKADREKYNRLARE WTQKYAM |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 D4E2 Ubiquitin-Conjugating Enzyme D4Hbuce1Ubiquitin Carrier Protein D4Ubiquitin-Protein Ligase D4 |
| Database Links | Reactome: R-HSA-983168 |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 D4 antibodyAnti-E2 Ubiquitin-Conjugating Enzyme D4 antibodyAnti-Hbuce1 antibodyAnti-Ubiquitin Carrier Protein D4 antibodyAnti-Ubiquitin-Protein Ligase D4 antibodyAnti-UBE2D4 antibodyAnti-UBCH5D antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/101832/390042/STJ28552_1__58226.1713159925.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
