| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBA1 |
| Gene ID: | 7317 |
| Uniprot ID: | UBA1_HUMAN |
| Immunogen Region: | 300-600 |
| Immunogen Sequence: | KISFKSLVASLAEPDFVVTD FAKFSRPAQLHIGFQALHQF CAQHGRPPRPRNEEDAAELV ALAQAVNARALPAVQQNNLD EDLIRKLAYVAAGDLAPINA FIGGLAAQEVMKACSGKFMP IMQWLYFDALECLPEDKEVL TEDKCLQRQNRYDGQVAVFG SDLQEKLGKQKYFLVGAGAI GCELLKNFAMIGLGCGEGGE IIVTDMDTIEKSNLNRQFLF RPWDVTKLKSDTAAAAVRQ |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human UBA1 (NP_695012.1). |
| Tissue Specificity | Detected in erythrocytes (at protein level). Ubiquitous. |
| Post Translational Modifications | ISGylated. |
| Function | Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites. |
| Protein Name | Ubiquitin-Like Modifier-Activating Enzyme 1Protein A1s9Ubiquitin-Activating Enzyme E1 |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmMitochondrionNucleusIsoform 1: NucleusIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin-Like Modifier-Activating Enzyme 1 antibodyAnti-Protein A1s9 antibodyAnti-Ubiquitin-Activating Enzyme E1 antibodyAnti-UBA1 antibodyAnti-A1S9T antibodyAnti-UBE1 antibody |
Information sourced from Uniprot.org
