• Immunofluorescence analysis of HeLa cells using UBA1 Rabbit polyclonal antibody (STJ114237) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of UBA1 in paraffin-embedded Mouse brain using UBA1 Rabbit polyclonal antibody (STJ114237) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of UBA1 in paraffin-embedded Human colon carcinoma using UBA1 Rabbit polyclonal antibody (STJ114237) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of UBA1 in paraffin-embedded Rat liver using UBA1 Rabbit polyclonal antibody (STJ114237) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using UBA1 Rabbit polyclonal antibody (STJ114237) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-UBA1 antibody (300-600) (STJ114237)

SKU:
STJ114237

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBA1 (300-600) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBA1
Gene ID: 7317
Uniprot ID: UBA1_HUMAN
Immunogen Region: 300-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human UBA1 (NP_695012.1).
Immunogen Sequence: KISFKSLVASLAEPDFVVTD FAKFSRPAQLHIGFQALHQF CAQHGRPPRPRNEEDAAELV ALAQAVNARALPAVQQNNLD EDLIRKLAYVAAGDLAPINA FIGGLAAQEVMKACSGKFMP IMQWLYFDALECLPEDKEVL TEDKCLQRQNRYDGQVAVFG SDLQEKLGKQKYFLVGAGAI GCELLKNFAMIGLGCGEGGE IIVTDMDTIEKSNLNRQFLF RPWDVTKLKSDTAAAAVRQ
Tissue Specificity Detected in erythrocytes (at protein level). Ubiquitous.
Post Translational Modifications ISGylated.
Function Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.
Protein Name Ubiquitin-Like Modifier-Activating Enzyme 1
Protein A1s9
Ubiquitin-Activating Enzyme E1
Database Links Reactome: R-HSA-8866652
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Mitochondrion
Nucleus
Isoform 1: Nucleus
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Ubiquitin-Like Modifier-Activating Enzyme 1 antibody
Anti-Protein A1s9 antibody
Anti-Ubiquitin-Activating Enzyme E1 antibody
Anti-UBA1 antibody
Anti-A1S9T antibody
Anti-UBE1 antibody

Information sourced from Uniprot.org

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