• Immunofluorescence analysis of U-2 OS cells using TXNRD1 antibody (STJ119056) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using TXNRD1 antibody (STJ119056) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using TXNRD1 antibody (STJ119056) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using TXNRD1 antibody (STJ119056) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human placenta using TXNRD1 antibody (STJ119056) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using TXNRD1 antibody (STJ119056) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-TXNRD1 antibody (STJ119056)

SKU:
STJ119056

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-TXNRD1 antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:100
IF: 1:50-1:200
Uniprot ID: TRXR1_HUMAN
Gene ID: 7296
Gene Symbol: TXNRD1
Immunogen: Recombinant protein of human TXNRD1
Tissue Specificity Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
Post Translational Modifications The N-terminus of isoform 5 is blocked. ISGylated.
Function Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
Protein Name Thioredoxin Reductase 1 - Cytoplasmic
Tr
Gene Associated With Retinoic And Interferon-Induced Mortality 12 Protein
Grim-12
Gene Associated With Retinoic And Ifn-Induced Mortality 12 Protein
Km-102-Derived Reductase-Like Factor
Thioredoxin Reductase Tr1
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2408550
Reactome: R-HSA-3299685
Reactome: R-HSA-499943
Reactome: R-HSA-5263617
Reactome: R-HSA-5336415
Reactome: R-HSA-5628897
Cellular Localisation Cytoplasm
Isoform 4: Cytoplasm
Nucleus
Isoform 5: Cytoplasm
Alternative Antibody Names Anti-Thioredoxin Reductase 1 - Cytoplasmic antibody
Anti-Tr antibody
Anti-Gene Associated With Retinoic And Interferon-Induced Mortality 12 Protein antibody
Anti-Grim-12 antibody
Anti-Gene Associated With Retinoic And Ifn-Induced Mortality 12 Protein antibody
Anti-Km-102-Derived Reductase-Like Factor antibody
Anti-Thioredoxin Reductase Tr1 antibody
Anti-TXNRD1 antibody
Anti-GRIM12 antibody
Anti-KDRF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance