| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Speckle targeted PIP5K1A-regulated polyA polymerase (291-340 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TUT1 |
| Gene ID: |
64852 |
| Uniprot ID: |
STPAP_HUMAN |
| Immunogen Region: |
291-340 aa |
| Specificity: |
TUTase Polyclonal Antibody detects endogenous levels of TUTase protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human TUT1 at the amino acid range 291-340 |
| Post Translational Modifications | Phosphorylated by CK1 in the proline-rich (Pro-rich) region. |
| Function | Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation. |
| Protein Name | Speckle Targeted Pip5k1a-Regulated Poly(A PolymeraseStar-PapRna-Binding Motif Protein 21Rna-Binding Protein 21U6 Snrna-Specific Terminal Uridylyltransferase 1U6-Tutase |
| Cellular Localisation | NucleusNucleolusNucleus Speckle |
| Alternative Antibody Names | Anti-Speckle Targeted Pip5k1a-Regulated Poly(A Polymerase antibodyAnti-Star-Pap antibodyAnti-Rna-Binding Motif Protein 21 antibodyAnti-Rna-Binding Protein 21 antibodyAnti-U6 Snrna-Specific Terminal Uridylyltransferase 1 antibodyAnti-U6-Tutase antibodyAnti-TUT1 antibodyAnti-RBM21 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
