• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g TUFM antibody (STJ28506). Western blot was performed from the immunoprecipitate using TUFM antibody (STJ28506) at a dilution of 1:1000.
  • Confocal immunofluorescence analysis of U2OS cells using TUFM Rabbit polyclonal antibody (STJ28506) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using TUFM Rabbit polyclonal antibody (STJ28506). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TUFM in paraffin-embedded human esophageal cancer using TUFM Rabbit polyclonal antibody (STJ28506) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TUFM Rabbit polyclonal antibody (STJ28506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TUFM antibody (186-455) (STJ28506)

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STJ28506

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TUFM (186-455) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:10-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TUFM
Gene ID: 7284
Uniprot ID: EFTU_HUMAN
Immunogen Region: 186-455
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3).
Immunogen Sequence: ADAVQDSEMVELVELEIREL LTEFGYKGEETPVIVGSALC ALEGRDPELGLKSVQKLLDA VDTYIPVPARDLEKPFLLPV EAVYSVPGRGTVVTGTLERG ILKKGDECELLGHSKNIRTV VTGIEMFHKSLERAEAGDNL GALVRGLKREDLRRGLVMVK PGSIKPHQKVEAQVYILSKE EGGRHKPFVSHFMPVMFSLT WDMACRIILPPEKELAMPGE DLKFNLILRQPMILEKGQR
Function Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Also plays a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.
Protein Name Elongation Factor Tu - Mitochondrial
Ef-Tu
P43
Database Links Reactome: R-HSA-5389840
Reactome: R-HSA-9754560
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Elongation Factor Tu - Mitochondrial antibody
Anti-Ef-Tu antibody
Anti-P43 antibody
Anti-TUFM antibody

Information sourced from Uniprot.org

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