| Short Description : | Rabbit polyclonal TUFM (186-455) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:10-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TUFM |
| Gene ID: | 7284 |
| Uniprot ID: | EFTU_HUMAN |
| Immunogen Region: | 186-455 |
| Immunogen Sequence: | ADAVQDSEMVELVELEIREL LTEFGYKGEETPVIVGSALC ALEGRDPELGLKSVQKLLDA VDTYIPVPARDLEKPFLLPV EAVYSVPGRGTVVTGTLERG ILKKGDECELLGHSKNIRTV VTGIEMFHKSLERAEAGDNL GALVRGLKREDLRRGLVMVK PGSIKPHQKVEAQVYILSKE EGGRHKPFVSHFMPVMFSLT WDMACRIILPPEKELAMPGE DLKFNLILRQPMILEKGQR |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3). |
| Function | GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Also plays a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy. |
| Protein Name | Elongation Factor Tu - MitochondrialEf-TuP43 |
| Database Links | Reactome: R-HSA-5389840Reactome: R-HSA-9754560 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Elongation Factor Tu - Mitochondrial antibodyAnti-Ef-Tu antibodyAnti-P43 antibodyAnti-TUFM antibody |
Information sourced from Uniprot.org
