• Immunofluorescence analysis of C6 cells using Beta II-Tubulin/Beta 2-Tubulin Rabbit monoclonal antibody (STJ11102157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Beta II-Tubulin/Beta 2-Tubulin Rabbit monoclonal antibody (STJ11102157) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Beta II-Tubulin/Beta 2-Tubulin Rabbit monoclonal antibody (STJ11102157) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Beta II-Tubulin/Beta 2-Tubulin Rabbit monoclonal antibody (STJ11102157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TUBB2A/TUBB2B antibody (346-445) [S7MR] (STJ11102157)

SKU:
STJ11102157

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Tubulin Beta 2 (346-445) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 346-445
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 346-445 of human Beta II-Tubulin/Beta 2-Tubulin (Q13885).
Immunogen Sequence: PNNVKTAVCDIPPRGLKMSA TFIGNSTAIQELFKRISEQF TAMFRRKAFLHWYTGEGMDE MEFTEAESNMNDLVSEYQQY QDATADEQGEFEEEEGEDEA
Background This gene encodes the receptor for the anti-Mullerian hormone (AMH) which, in addition to testosterone, results in male sex differentiation. AMH and testosterone are produced in the testes by different cells and have different effects. Testosterone promotes the development of male genitalia while the binding of AMH to the encoded receptor prevents the development of the mullerian ducts into uterus and Fallopian tubes. Mutations in this gene are associated with persistent Mullerian duct syndrome type II. Alternatively spliced transcript variants encoding different isoforms have been identified.

Information sourced from Uniprot.org

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