| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Trna (1-250) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRMT2A |
| Gene ID: |
27037 |
| Uniprot ID: |
TRM2A_HUMAN |
| Immunogen Region: |
1-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT2A/HTF9C (NP_073564.3). |
| Immunogen Sequence: |
MSENLDNEGPKPMESCGQES SSALSCPTVSVPPAAPAALE EVEKEGAGAATGPGPQPGLY SYIRDDLFTSEIFKLELQNV PRHASFSDVRRFLGRFGLQP HKTKLFGQPPCAFVTFRSAA ERDKALRVLHGALWKGRPLS VRLARPKADPMARRRRQEGE SEPPVTRVADVVTPLWTVPY AEQLERKQLECEQVLQKLAK EIGSTNRALLPWLLEQRHKH NKACCPLEGVRPSPQQTEY |
| Function | S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs. Mainly catalyzes the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs. Also able to mediate the formation of 5-methyl-uridine in some mRNAs. |
| Protein Name | TrnaUracil-5--Methyltransferase Homolog AMrnaUracil-5--Methyltransferase Trmt2a |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Uracil-5--Methyltransferase Homolog A antibodyAnti-Mrna antibodyAnti-Uracil-5--Methyltransferase Trmt2a antibodyAnti-TRMT2A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100805) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89741/358216/STJ11100805_1__91183.1713122541.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100805) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100863) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89797/358266/STJ11100863_1__40910.1713122607.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100863) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
